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Biochemistry

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JoVE Journal Biochemistry
Metod för att identifiera små molekylhämmare av protein-proteininteraktion Mellan HCN1 och TRIP8b
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Metod för att identifiera små molekylhämmare av protein-proteininteraktion Mellan HCN1 och TRIP8b

Article DOI: 10.3791/54540-v • 10:20 min • November 11th, 2016
November 11th, 2016 •
*1, *1, 2, 3, 1, 1, 4, 1,5
1Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery, Northwestern University, 3Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, 5Department of Physiology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Samspelet mellan HCN kanaler och deras hjälp subenhet har identifierats som ett terapeutiskt mål i egentlig depression. Här, en fluorescenspolariserings-baserad metod för att identifiera småmolekylära hämmare av detta protein-proteininteraktion, presenteras.

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Biokemi High-throughput screening läkemedelsutveckling protein-proteininteraktioner fluorescenspolarisation HCN TRIP8b
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