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Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

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This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.

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Neuronal Viability Fluorescence Staining Double Staining Cerebellar Granule Neuron Culture Cell Viability Measurement Neuroscience Neuropharmacology Glial Cells MISTA Cell Cultures Rat Pups Dissection Forceps Meninges Blood Vessels Centrifuge Tube Trypsin Solution DNase One
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