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JoVE Journal Biochemistry
Rensing av biotinylerte celleoverflateproteiner fra
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Rensing av biotinylerte celleoverflateproteiner fra

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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En modifiserings-sentrifugeringsgradientbasert metodikk ble benyttet for å isolere epitelceller fra Rhipicephalus microplus gut-vev. Overflatbundne proteiner ble biotinylert og renset gjennom streptavidinmagnetiske perler for utnyttelse i nedstrømsapplikasjoner.

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Biokjemi utgave 125 celleoverflateproteiner biotinylering flått mikroplus tarmepitelceller
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