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Journal / Biochemistry / Rening av biotinylerade cellytaproteiner från
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JoVE Journal Biochemistry
Rening av biotinylerade cellytaproteiner från
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Rening av biotinylerade cellytaproteiner från

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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En modifierad densitetscentrifugeringsgradientbaserad metod användes för att isolera epitelceller från Rhipicephalus microplus darmvävnad. Ytbundna proteiner biotinylerades och renades genom streptavidinmagnetiska pärlor för användning vid nedströmsapplikationer.

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Biochemistry Cell-ytproteiner biotinylering fästingar microplus tarm-epitelceller
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