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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Capítulos
- 00:04Título
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
Vi rapporterer en lille hårnål RNA (shRNA) og næste generation sequencing-baseret protokol til at identificere regulatorer af x-kromosom Inaktiveringen i en murine cellelinie med firefly luciferase og hygromycin resistensgener smeltet til methyl CpG bindende protein 2) MeCP2) gen på det inaktive X kromosom.
