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JoVE Journal Developmental Biology
Equivalenti di tessuto umano infiammatorio tridimensionale del Gingiva
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Equivalenti di tessuto umano infiammatorio tridimensionale del Gingiva

Article DOI: 10.3791/57157-v • 08:43 min • April 3rd, 2018
April 3rd, 2018 •
1, 2, 2
1Department of Pharmacology, The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital

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L'obiettivo del protocollo è quello di costruire un modello di infiammatorio gengiva umana in vitro. Questo modello di tessuto Co-coltiva tre tipi di cellule umane, keratinocytes di HaCaT, fibroblasti gengivali e macrofagi THP-1, in condizioni tridimensionali. Questo modello può essere applicato a indagare le malattie parodontali, quali gengivite e parodontite.

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Biologia dello sviluppo problema 134 cultura tridimensionale equivalenti di gengiva fibroblasti gengivali umani cellule HaCaT cellule THP-1 macrofagi ingegneria tissutale modello di tessuto infiammatorio gengivite parodontite la guarigione della ferita tessuto rigenerazione
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