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Journal / Immunology and Infection / 腹膜源性肥大细胞的分离及其功能特征的 Ca2…
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Immunology and Infection

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JoVE Journal Immunology and Infection
腹膜源性肥大细胞的分离及其功能特征的 Ca2 +成像和脱粒测定
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腹膜源性肥大细胞的分离及其功能特征的 Ca2 +成像和脱粒测定

Article DOI: 10.3791/57222-v • 11:31 min • July 4th, 2018
July 4th, 2018 •
1, 1, 1, 1
1Institute of Pharmacology, Ruprecht-Karls Heidelberg University

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在这里, 我们提出了一个隔离和培养小鼠腹膜肥大细胞的协议。我们还描述了两个协议的功能表征: 一个荧光成像的细胞内游离 Ca2 +浓度和脱粒检测的基础上的色度量化的释放β-hexosaminidase。

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免疫学和感染 问题 137 腹膜肥大细胞 小鼠肥大细胞 胞内游离钙浓度 荧光成像 脱粒 调解人释放 β-hexosaminidase
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