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Generation af menneskers Nasal epitelcelle Spheroids for individualiseret cystisk fibrose Transmembrane ledningsevne Regulator undersøgelse

Article DOI: 10.3791/57492-v • 08:00 min • April 11th, 2018
April 11th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Pediatrics, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center

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Her beskriver vi en metode til at generere tredimensionelle klumpformet kulturer af menneskers nasal epitelceller. Spheroids derefter stimuleres til at drive cystisk fibrose Transmembrane ledningsevne Regulator (CFTR)-afhængige ion og væske sekretion, kvantificere ændringen i klumpformet luminale størrelse som en proxy for CFTR funktion.

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Medicin spørgsmål 134 cystisk fibrose CFTR Precision medicin Organoid sfæroide Nasal celle cellekultur
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