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Developmental Biology

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JoVE Journal Developmental Biology
Generation av byggnadsställning-fri, tredimensionella Insulin att uttrycka Pancreatoids från mus bukspottskörteln föräldraparets In Vitro
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Generation av byggnadsställning-fri, tredimensionella Insulin att uttrycka Pancreatoids från mus bukspottskörteln föräldraparets In Vitro

Article DOI: 10.3791/57599-v • 09:33 min • June 2nd, 2018
June 2nd, 2018 •
1, 2,3,4, 2,3,4, 1,2,3,4,5
1Program in Developmental Biology, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, 3Molecular and Cellular Biology Department, Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, 5McNair Medical Institute, Baylor College of Medicine

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Här presenterar vi ett protokoll för att generera insulin uttrycker 3D murina pancreatoids från friflytande e10.5 dissocierade bukspottskörteln stamfäder och den associerade mesenchyme.

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Utvecklingsbiologi fråga 136 bukspottkörteln Organoids betaceller utveckling Mesenchyme differentiering Diabetes 3D kultur Pancreatoids
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