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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Canini trappola extracellulari del neutrofilo formazione
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
Journal (Immunology and Infection)
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Journal (Immunology and Infection)
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
 
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In Vitro Canini trappola extracellulari del neutrofilo formazione: Analisi dinamica e quantitativa mediante microscopia a fluorescenza

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Descriviamo i metodi per isolare canini neutrofili dal sangue intero e visualizzare formazione netta nei neutrofili dal vivo utilizzando la microscopia a fluorescenza. Sono anche descritti protocolli per quantificare la formazione netta e citrullinato istone H3 (citH3) espressione usando la microscopia di immunofluorescenza.

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Immunologia e infezione problema 138 microscopia di immunofluorescenza sepsi isolamento del granulocyte istone citrullination peptidylarginine deiminasi
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