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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Canine neutrofile ekstracellulære fælde dannelse
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In Vitro Canine neutrofile ekstracellulære fælde dannelse: Dynamisk og kvantitativ analyse af Fluorescens mikroskopi

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Vi beskriver metoder til at isolere canine neutrofiler fra fuldblod og visualisere netto dannelse i live neutrofiler ved hjælp af Fluorescens mikroskopi. Også beskrevet er protokoller at kvantificere netto dannelse og citrullinated Histon H3 (citH3) udtryk ved hjælp af immunofluorescens mikroskopi.

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Immunologi og infektion spørgsmålet 138 immunofluorescens mikroskopi sepsis granulocyt isolation Histon citrullinering peptidylarginine deiminase
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