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JoVE Journal Neuroscience
Voorbereiding van Acute ruggenmerg segmenten geheel-cel Patch-clamp opname in neuronen van de Substantia Gelatinosa
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Voorbereiding van Acute ruggenmerg segmenten geheel-cel Patch-clamp opname in neuronen van de Substantia Gelatinosa

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Hier beschrijven we de essentiële stappen voor geheel-cel patch-clamp opnamen gemaakt van substantia gelatinosa (SG) neuronen in het ruggenmerg in vitro segment. Met deze methode kunt de eigenschappen van het intrinsieke membraan, synaptische transmissie en morfologische karakterisering van SG neuronen worden bestudeerd.

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Neurowetenschappen kwestie 143 neurowetenschap ruggenmerg slice substantia gelatinosa neuron geheel-cel patch-clamp elektrofysiologie morfologie in vitro
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