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Lyddæmpnings spark: CRISPR/Cas9 Genome redigering i svagt elektrisk fisk

Article DOI: 10.3791/60253-v • 08:00 min • October 27th, 2019
October 27th, 2019 •
1, 2, 2, 3, 1
1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University

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Her præsenteres en protokol for at producere og bageste crispr/Cas9 genom knockout elektrisk fisk. Detaljeret beskrevet er de nødvendige molekylær biologi, avl og opdræts krav for både en gymnotiform og en mormyrid, og injektion teknikker til at producere Cas9-induceret Indel F0 larver.

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Biologi CRISPR/Cas9 elektriske fisk enkelt celle mikroinjektion scn4aa elektrisk organ udledning genom modifikation mormyrid gymnotiform
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