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Dämpning av gnistan: CRISPR/Cas9 genom redigering i svagt elektrisk fisk

Article DOI: 10.3791/60253-v • 08:00 min • October 27th, 2019
October 27th, 2019 •
1, 2, 2, 3, 1
1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University

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Här presenteras ett protokoll för att producera och bakre CRISPR/Cas9 Genome knockout elektrisk fisk. Beskrivs i detalj är de nödvändiga molekylära biologi, avel och djurhållning krav för både en gymnotiform och en mormyrid, och injektion tekniker för att producera Cas9-inducerad indel F0 larver.

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Biologi CRISPR/Cas9 elektrisk fisk encellig mikroinjektion scn4aa elektriskt organ urladdning arvsmassa modifiering mormyrid gymnotiform
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