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Bioengineering

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JoVE Journal Bioengineering
Høj gennemløb Gær Strain Fænotryk med Dråbe-baseret RNA Sekventering
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Høj gennemløb Gær Strain Fænotryk med Dråbe-baseret RNA Sekventering

Article DOI: 10.3791/61014-v • 07:55 min • May 21st, 2020
May 21st, 2020 •
1,2, 1, 1, 3,5, 1,4,5
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 2University of California Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, 3Department of Pharmaceutical Chemistry, University of California San Francisco, 4California Institute for Quantitative Biosciences, University of California San Francisco, 5Chan Zuckerberg Biohub

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En flaskehals i den "design-build-test" cyklus af mikrobiel teknik er den hastighed, hvormed vi kan udføre funktionelle skærme af stammer. Vi beskriver en høj-throughput metode til stamme screening anvendes til hundreder til tusindvis af gær celler pr eksperiment, der udnytter dråbe-baserede RNA sekventering.

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Bioengineering mikrobiel teknik gærstammer enkeltcelleRNA-sekvensering dråbemikrofluidics sekvensering med høj gennemløb hydrogeldråber
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