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JoVE Journal Biochemistry
Fjernelse og udskiftning af endogene ligands fra Lipid-Bound Proteiner og allergener
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Fjernelse og udskiftning af endogene ligands fra Lipid-Bound Proteiner og allergener

Article DOI: 10.3791/61780-v • 09:09 min • February 24th, 2021
February 24th, 2021 •
1, 1, 1
1Nuclear Magnetic Resonance Group, National Institute of Environmental Health Sciences

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Denne protokol beskriver fjernelsen af endogene lipider fra allergener og deres erstatning med brugerspecifikke ligands gennem omvendt fase HPLC kombineret med termisk udglødning. 31 år P-NMR og cirkulær dichroism giver mulighed for hurtig bekræftelse af ligand fjernelse / lastning, og inddrivelse af indfødte allergen struktur.

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Biokemi Problem 168 allergener biokemi biofysik fedtsyrebindende proteiner fedtsyrer lipider proteinbinding proteinstabilitet
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