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Journal / Biochemistry / Oprensning af endogene drosophila transiente…
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JoVE Journal Biochemistry
Oprensning af endogene drosophila transiente receptorpotentialekanaler
Fixed Target Serial Data Collection at Diamond Light Source
Journal (Biochemistry)
Fixed Target Serial Data Collection at Diamond Light Source
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Journal (Biochemistry)
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
 
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Oprensning af endogene drosophila transiente receptorpotentialekanaler

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Baseret på samlingsmekanismen for INAD-proteinkomplekset blev der i denne protokol udviklet en modificeret affinitetsrensning plus konkurrencestrategi for at rense den endogene Drosophila TRP-kanal.

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Biokemi udgave 178 proteinrensning Drosophila TRP-kanal INAD
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