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Biochemistry

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JoVE Journal Biochemistry
Spot variation fluorescens korrelationsspektroskopi til analyse af molekylær diffusion ved plasmamembranen af levende celler
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Spot variation fluorescens korrelationsspektroskopi til analyse af molekylær diffusion ved plasmamembranen af levende celler

Article DOI: 10.3791/61823-v • 05:56 min • November 12th, 2020
November 12th, 2020 •
*1, *2, *1, 3, 4, 2, 1, 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ
* These authors contributed equally

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Denne artikel har til formål at præsentere en protokol om, hvordan man bygger et spotvariation Fluorescence Correlation Spectroscopy (svFCS) mikroskop til måling af molekylær diffusion ved plasmamembranen i levende celler.

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Biokemi udgave 165 spotvariation Fluorescens Korrelationsspektroskopi (svFCS) lateral diffusion plasmamembrandynamik molekylær indeslutning lipidnanodomæner cytoskelet meshwork diffusionslov
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