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Isolering av mus interstitial ventilceller for å studere forkalkning av aortaventilen in vitro

Article DOI: 10.3791/62419-v • 05:47 min • May 10th, 2021
May 10th, 2021 •
1, 1,2,3
1Cardiovascular Research Center, The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine, The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences, The Icahn School of Medicine at Mount Sinai

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Denne artikkelen beskriver isolering av museaortaventilceller ved hjelp av en to-trinns kollagenalensprosedyre. Isolerte museventilceller er viktige for å utføre forskjellige analyser, for eksempel denne in vitro-forkalkningsanalysen, og for å undersøke de molekylære veiene som fører til aortaventilmineralisering.

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