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Rektal Organoid Morfoloji Analizi (ROMA): Kistik Fibroziste Tanısal Bir Tahlil

Article DOI: 10.3791/63818-v • 07:56 min • June 10th, 2022
June 10th, 2022 •
1,2, 1, 3,4, 5, 6, 7,8, 7,9, 8, 3,4, 1,2, 1,2, 10,11, 1,2, 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

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Bu protokol, kistik fibroz (KF) için yeni bir tanısal test olan rektal organoid morfoloji analizini (ROMA) tanımlamaktadır. Morfolojik özellikler, yani yuvarlaklık (dairesellik indeksi, CI) ve bir lümenin varlığı (yoğunluk oranı, IR), CFTR fonksiyonunun bir ölçüsüdür. 189 deneğin analizi, CF ve CF olmayan arasında mükemmel bir ayrım olduğunu göstermiştir.

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