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Co-culture et transduction de thymocytes murins sur des cellules stromales exprimant 4-Delta-like pour étudier les oncogènes dans la leucémie à cellules T
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Co-culture et transduction de thymocytes murins sur des cellules stromales exprimant 4-Delta-like pour étudier les oncogènes dans la leucémie à cellules T

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Ce protocole décrit l’isolement de thymocytes doublement négatifs du thymus de souris, suivi d’une transduction rétrovirale et d’une co-culture sur le système de co-culture de lignée cellulaire stromale de moelle osseuse de type delta 4-exprimant la moelle osseuse (OP9-DL4) pour une analyse fonctionnelle plus approfondie.

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Cancer Research numéro 196 Thymocyte OP9 lymphopoïèses transduction co-culture
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