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JoVE Journal Cancer Research
Samkultur og transduksjon av murine tymocytter på delta-lignende 4-uttrykkende stromale celler for å studere onkogener i T-celle leukemi
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Samkultur og transduksjon av murine tymocytter på delta-lignende 4-uttrykkende stromale celler for å studere onkogener i T-celle leukemi

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Denne protokollen beskriver isolering av dobbeltnegative tymocytter fra musens thymus etterfulgt av retroviral transduksjon og kokultur på det deltalignende 4-uttrykkende benmargsstromale cellelinjedyrkningssystemet (OP9-DL4) for videre funksjonell analyse.

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Kreftforskning utgave 196 Thymocyte OP9 lymfopoieser transduksjon co-kultur
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