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Immunology and Infection

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JoVE Journal Immunology and Infection
Effiziente Transfektion von in vitro transkribierter mRNA in kultivierten Zellen mit Peptid-Poloxamin-Nanopartikeln
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Journal (Immunology and Infection)
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Journal (Immunology and Infection)
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Effiziente Transfektion von in vitro transkribierter mRNA in kultivierten Zellen mit Peptid-Poloxamin-Nanopartikeln

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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Ein selbstorganisiertes Peptid-Poloxamin-Nanopartikel (PP-sNp) wird unter Verwendung einer mikrofluidischen Mischvorrichtung entwickelt, um in vitro transkribierte Boten-RNA zu verkapseln und zu liefern. Die beschriebene mRNA/PP-sNp konnte kultivierte Zellen in vitro effizient transfizieren.

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Immunologie und Infektion Ausgabe 186 Genabgabe in vitro transkribierte Boten-RNA Abgabesysteme Poloxamin Nanopartikel
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