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JoVE Journal Biochemistry
Optimering af flowcytometriske sorteringsparametre til high-throughput isolering og oprensning af små ekstracellulære vesikler
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Optimering af flowcytometriske sorteringsparametre til high-throughput isolering og oprensning af små ekstracellulære vesikler

Article DOI: 10.3791/64360-v • 10:16 min • January 20th, 2023
January 20th, 2023 •
1, 2, 3, 3, 3, 3, 3, 2
1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine

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Denne protokol giver en hurtig og størrelsesspecifik isoleringsmetode til små ekstracellulære vesikler ved at optimere størrelsen på luftsprøjtedysen, kappevæsketrykket, prøveflowtrykket, spændingen, forstærkningen og udløsningstærskelparametrene.

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Tilbagetrækning nr. 191
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