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A Simple Protocol for Mapping the Plant Root System Architecture Traits

Article DOI: 10.3791/64876-v • 11:09 min • February 10th, 2023
February 10th, 2023 •
1,2, 2,3, 1,4
1Department of Biology, Western Kentucky University, 2Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, 3Academy of Scientific and Innovative Research (AcSIR), 4Department of Biology, St. Joseph's University

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We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

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Non-invasive Hydroponic Method Root System Architecture Lab Equipment Visualization RSA Analysis Elemental Contamination Environmental Interaction Hormonal Conditions Nutrient Conditions Climactic Conditions Surface Sterilization Arabidopsis Seeds Distilled Water Centrifuge Ethanol Treatment Sterile Water Commercial Bleach TWEEN 20
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