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An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
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An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Article DOI: 10.3791/51530-v • 09:33 min • June 19th, 2014
June 19th, 2014 •
1, 2, 1
1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, 2Institut de Recherche pour le Développement (UMR 232), Centre National de la Recherche Scientifique (ERL 5300), Université de Montpellier II

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We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear architecture.

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Quantitative Single-cell Analysis Chromatin Modification Nuclear Architecture Whole-mount Ovules Arabidopsis Spore Mother Cells Megaspore Mother Cell MMC Meiosis Mitosis Female Gametophyte Egg Cell Central Cell Accessory Cells Cytological Analysis Cytogenetic Analysis Immunodetection Cellular Epitopes Nuclear Epitopes Penetration Of Reagents Single-cell Imaging Optical Clarity Whole-mount Tissues Nuclear Organization High Resolution Analysis Acrylamide Gel Embedding
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