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Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Article DOI: 10.3791/914-v • 10:59 min • October 1st, 2008
October 1st, 2008 •
1, 1
1Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School

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The cellular heterogeneity of brain tissue poses a significant limitation for the study of epigenetic markings in chromatin because most assays lack single cell resolution. Neurons typically are intermingled with glia and other non-neuronal cells. We provide a protocol to extract and collect neuronal nuclei from human brain.

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Neuronal Nuclei Isolation Human Postmortem Brain Tissue Neuronal Chromatin Nuclear Organization Chromatin And DNA-based Assays Single Cell Resolution Cellular Heterogeneity Glia Cells Non-neuronal Cells Cell-type Specific Cultures CNS Cells Epigenetic Mechanisms Frozen Brain Tissue Hypotonic Lysis Buffer Ultracentrifugation Immunotagging Anti-NeuN Antibody Fluorescence-activated Sorting Chromatin Immunoprecipitation Studies
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