Wolfgang Wiechert IBG-1: Biotechnology Forschungszentrum Jülich Biography Publications Institution JoVE Articles Wolfgang Wiechert has not added a biography. If you are Wolfgang Wiechert and would like to personalize this page please email our Author Liaison for assistance. Publications Automated Growth Rate Determination in High-throughput Microbioreactor Systems BMC Research Notes. Nov, 2017 | Pubmed ID: 29178966 Laboratory Scale Photobiotechnology - Current Trends and Future Perspectives FEMS Microbiology Letters. Nov, 2017 | Pubmed ID: 29126108 PH Fluctuations Imperil the Robustness of C. Glutamicum to Short Term Oxygen Limitation Journal of Biotechnology. Oct, 2017 | Pubmed ID: 28837821 Corynebacterium Glutamicum Chassis C1*: Building and Testing a Novel Platform Host for Synthetic Biology and Industrial Biotechnology ACS Synthetic Biology. Aug, 2017 | Pubmed ID: 28803482 To Be Certain About the Uncertainty: Bayesian Statistics for 13 C Metabolic Flux Analysis Biotechnology and Bioengineering. Nov, 2017 | Pubmed ID: 28695999 Homogenizing Bacterial Cell Factories: Analysis and Engineering of Phenotypic Heterogeneity Metabolic Engineering. Jul, 2017 | Pubmed ID: 28645641 Miniaturized and Automated Adaptive Laboratory Evolution: Evolving Corynebacterium Glutamicum Towards an Improved D-xylose Utilization Bioresource Technology. Dec, 2017 | Pubmed ID: 28552568 Fast and Reliable Strain Characterization of Streptomyces Lividans Through Micro-scale Cultivation Biotechnology and Bioengineering. Sep, 2017 | Pubmed ID: 28436005 Coarse-graining Bacteria Colonies for Modelling Critical Solute Distributions in Picolitre Bioreactors for Bacterial Studies on Single-cell Level Microbial Biotechnology. Jul, 2017 | Pubmed ID: 28371389 A Framework for Accelerated Phototrophic Bioprocess Development: Integration of Parallelized Microscale Cultivation, Laboratory Automation and Kriging-assisted Experimental Design Biotechnology for Biofuels. 2017 | Pubmed ID: 28163783 Comparative Evaluation of Phototrophic Microtiter Plate Cultivation Against Laboratory-scale Photobioreactors Bioprocess and Biosystems Engineering. May, 2017 | Pubmed ID: 28101644 Metabolic Profile of 1,5-diaminopentane Producing Corynebacterium Glutamicum Under Scale-down Conditions: Blueprint for Robustness to Bioreactor Inhomogeneities Biotechnology and Bioengineering. Mar, 2017 | Pubmed ID: 27641904 Design and Validation of a Parallelized Micro-photobioreactor Enabling Phototrophic Bioprocess Development at Elevated Throughput Biotechnology and Bioengineering. Jan, 2017 | Pubmed ID: 27424867 Use of a Sec Signal Peptide Library from Bacillus Subtilis for the Optimization of Cutinase Secretion in Corynebacterium Glutamicum Microbial Cell Factories. Dec, 2016 | Pubmed ID: 27927208 A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid L-Lysine Analysis Sensors (Basel, Switzerland). Sep, 2016 | Pubmed ID: 27690044 Image-Based Single Cell Profiling: High-Throughput Processing of Mother Machine Experiments PloS One. 2016 | Pubmed ID: 27661996 Time-resolved, Single-cell Analysis of Induced and Programmed Cell Death Via Non-invasive Propidium Iodide and Counterstain Perfusion Scientific Reports. Sep, 2016 | Pubmed ID: 27580964 μMORE: A Microfluidic Magnetic Oscillation Reactor for Accelerated Parameter Optimization in Biocatalysis Journal of Biotechnology. Aug, 2016 | Pubmed ID: 27288595 Automation of a Nile Red Staining Assay Enables High Throughput Quantification of Microalgal Lipid Production Microbial Cell Factories. Feb, 2016 | Pubmed ID: 26861538 Discrete-continuous Reaction-diffusion Model with Mobile Point-like Sources and Sinks The European Physical Journal. E, Soft Matter. Jan, 2016 | Pubmed ID: 26830760 A Scientific Workflow Framework for (13)C Metabolic Flux Analysis Journal of Biotechnology. Aug, 2016 | Pubmed ID: 26721184 Photocaged Arabinose: A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression Chembiochem : a European Journal of Chemical Biology. Feb, 2016 | Pubmed ID: 26677142 Cutting the Gordian Knot: Identifiability of Anaplerotic Reactions in Corynebacterium Glutamicum by Means of (13) C-metabolic Flux Analysis Biotechnology and Bioengineering. Mar, 2016 | Pubmed ID: 26375179 Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester PloS One. 2015 | Pubmed ID: 26513257 (13)C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter Oxydans 621H Metabolites. Sep, 2015 | Pubmed ID: 26404385 Spatiotemporal Microbial Single-cell Analysis Using a High-throughput Microfluidics Cultivation Platform Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Dec, 2015 | Pubmed ID: 26348020 Modeling and CFD Simulation of Nutrient Distribution in Picoliter Bioreactors for Bacterial Growth Studies on Single-cell Level Lab on a Chip. Nov, 2015 | Pubmed ID: 26345659 Vizardous: Interactive Analysis of Microbial Populations with Single Cell Resolution Bioinformatics (Oxford, England). Dec, 2015 | Pubmed ID: 26261223 Live Cell Imaging of SOS and Prophage Dynamics in Isogenic Bacterial Populations Molecular Microbiology. Nov, 2015 | Pubmed ID: 26235130 The Effect of Composition on Diffusion of Macromolecules in a Crowded Environment Physical Biology. May, 2015 | Pubmed ID: 26020120 Bioprocess Automation on a Mini Pilot Plant Enables Fast Quantitative Microbial Phenotyping Microbial Cell Factories. Mar, 2015 | Pubmed ID: 25888907 How to Measure Metabolic Fluxes: a Taxonomic Guide for (13)C Fluxomics Current Opinion in Biotechnology. Aug, 2015 | Pubmed ID: 25531408 Absolute Quantification of Corynebacterium Glutamicum Glycolytic and Anaplerotic Enzymes by QconCAT Journal of Proteomics. Jan, 2015 | Pubmed ID: 25451015 Visual Workflows for 13C-metabolic Flux Analysis Bioinformatics (Oxford, England). Feb, 2015 | Pubmed ID: 25297067 Chassis Organism from Corynebacterium Glutamicum--a Top-down Approach to Identify and Delete Irrelevant Gene Clusters Biotechnology Journal. Feb, 2015 | Pubmed ID: 25139579 Engineering of Corynebacterium Glutamicum for Minimized Carbon Loss During Utilization of D-xylose Containing Substrates Journal of Biotechnology. Dec, 2014 | Pubmed ID: 25304460 An Evaluation of Genetically Encoded FRET-based Biosensors for Quantitative Metabolite Analyses in Vivo Journal of Biotechnology. Dec, 2014 | Pubmed ID: 25107505 Rapid Assessment of Oxygen Transfer Impact for Corynebacterium Glutamicum Bioprocess and Biosystems Engineering. Dec, 2014 | Pubmed ID: 24981020 Quantitative Metabolomics: a Phantom? Trends in Biotechnology. May, 2014 | Pubmed ID: 24708998 Developing a New Production Host from a Blueprint: Bacillus Pumilus As an Industrial Enzyme Producer Microbial Cell Factories. Mar, 2014 | Pubmed ID: 24661794 Single-cell Microfluidics: Opportunity for Bioprocess Development Current Opinion in Biotechnology. Oct, 2014 | Pubmed ID: 24642389 Process Inhomogeneity Leads to Rapid Side Product Turnover in Cultivation of Corynebacterium Glutamicum Microbial Cell Factories. Jan, 2014 | Pubmed ID: 24410842 Assessment of Robustness Against Dissolved Oxygen/substrate Oscillations for C. Glutamicum DM1933 in Two-compartment Bioreactor Bioprocess and Biosystems Engineering. Nov, 2013 | Pubmed ID: 24218302 Microfluidic Growth Chambers with Optical Tweezers for Full Spatial Single-cell Control and Analysis of Evolving Microbes Journal of Microbiological Methods. Sep, 2013 | Pubmed ID: 24041615 Beyond Growth Rate 0.6: What Drives Corynebacterium Glutamicum to Higher Growth Rates in Defined Medium Biotechnology and Bioengineering. Aug, 2013 | Pubmed ID: 23996851 13C-flux Spectral Analysis of Host-pathogen Metabolism Reveals a Mixed Diet for Intracellular Mycobacterium Tuberculosis Chemistry & Biology. Aug, 2013 | Pubmed ID: 23911587 Simultaneous Utilization of Glucose and Gluconate in Penicillium Chrysogenum During Overflow Metabolism Biotechnology and Bioengineering. Dec, 2013 | Pubmed ID: 23775209 Isotopically Non-stationary Metabolic Flux Analysis: Complex Yet Highly Informative Current Opinion in Biotechnology. Dec, 2013 | Pubmed ID: 23623747 Metabolic Isotopomer Labeling Systems. Part III: Path Tracing Mathematical Biosciences. Jul, 2013 | Pubmed ID: 23507460 Combined Fluxomics and Transcriptomics Analysis of Glucose Catabolism Via a Partially Cyclic Pentose Phosphate Pathway in Gluconobacter Oxydans 621H Applied and Environmental Microbiology. Apr, 2013 | Pubmed ID: 23377928 13CFLUX2--high-performance Software Suite for (13)C-metabolic Flux Analysis Bioinformatics (Oxford, England). Jan, 2013 | Pubmed ID: 23110970 Beyond Growth Rate 0.6: Corynebacterium Glutamicum Cultivated in Highly Diluted Environments Biotechnology and Bioengineering. Jan, 2013 | Pubmed ID: 22890752 Error Propagation Analysis for Quantitative Intracellular Metabolomics Metabolites. Nov, 2012 | Pubmed ID: 24957773 Influence of Organic Solvents on Enzymatic Asymmetric Carboligations Advanced Synthesis & Catalysis. Oct, 2012 | Pubmed ID: 23349644 Extensive Exometabolome Analysis Reveals Extended Overflow Metabolism in Various Microorganisms Microbial Cell Factories. 2012 | Pubmed ID: 22963408 A Disposable Picolitre Bioreactor for Cultivation and Investigation of Industrially Relevant Bacteria on the Single Cell Level Lab on a Chip. May, 2012 | Pubmed ID: 22511122 Tailoring a Stabilized Variant of Hydroxynitrile Lyase from Arabidopsis Thaliana Chembiochem : a European Journal of Chemical Biology. Apr, 2012 | Pubmed ID: 22378361 Collisional Fragmentation of Central Carbon Metabolites in LC-MS/MS Increases Precision of ¹³C Metabolic Flux Analysis Biotechnology and Bioengineering. Mar, 2012 | Pubmed ID: 22012626 Optical Sensors for Monitoring Dynamic Changes of Intracellular Metabolite Levels in Mammalian Cells Nature Protocols. Nov, 2011 | Pubmed ID: 22036884 Comparative 13C Metabolic Flux Analysis of Pyruvate Dehydrogenase Complex-deficient, L-valine-producing Corynebacterium Glutamicum Applied and Environmental Microbiology. Sep, 2011 | Pubmed ID: 21784914 The Benefits of Being Transient: Isotope-based Metabolic Flux Analysis at the Short Time Scale Applied Microbiology and Biotechnology. Sep, 2011 | Pubmed ID: 21732247 Visualizing Multi-omics Data in Metabolic Networks with the Software Omix: a Case Study Bio Systems. Aug, 2011 | Pubmed ID: 21575673 Mechanistic Pathway Modeling for Industrial Biotechnology: Challenging but Worthwhile Current Opinion in Biotechnology. Oct, 2011 | Pubmed ID: 21353523 Stationary Versus Non-stationary (13)C-MFA: a Comparison Using a Consistent Dataset Journal of Biotechnology. Jul, 2011 | Pubmed ID: 20638432 Modeling Languages for Biochemical Network Simulation: Reaction Vs Equation Based Approaches Advances in Biochemical Engineering/biotechnology. 2010 | Pubmed ID: 20309675 Translating Biochemical Network Models Between Different Kinetic Formats Metabolic Engineering. Mar, 2009 | Pubmed ID: 19013536 13C Labeling Experiments at Metabolic Nonstationary Conditions: an Exploratory Study BMC Bioinformatics. 2008 | Pubmed ID: 18366666 Visualizing Regulatory Interactions in Metabolic Networks BMC Biology. 2007 | Pubmed ID: 17939866 The Topology of Metabolic Isotope Labeling Networks BMC Bioinformatics. 2007 | Pubmed ID: 17727715 The Thermodynamic Meaning of Metabolic Exchange Fluxes Biophysical Journal. Sep, 2007 | Pubmed ID: 17526563 Fluxomics: Mass Spectrometry Versus Quantitative Imaging Current Opinion in Plant Biology. Jun, 2007 | Pubmed ID: 17481942 Nondestructive and Noncontact Method for Determining the Spring Constant of Rectangular Cantilevers The Review of Scientific Instruments. Apr, 2007 | Pubmed ID: 17477668 Metabolic Flux Analysis at Ultra Short Time Scale: Isotopically Non-stationary 13C Labeling Experiments Journal of Biotechnology. Apr, 2007 | Pubmed ID: 17207877 Computational Tools for Isotopically Instationary 13C Labeling Experiments Under Metabolic Steady State Conditions Metabolic Engineering. Nov, 2006 | Pubmed ID: 16890470 Experimental Design Principles for Isotopically Instationary 13C Labeling Experiments Biotechnology and Bioengineering. Jun, 2006 | Pubmed ID: 16598793 Interpretation of Metabolic Flux Maps by Limitation Potentials and Constrained Limitation Sensitivities Biotechnology and Bioengineering. Jun, 2006 | Pubmed ID: 16596665 Emerging Corynebacterium Glutamicum Systems Biology Journal of Biotechnology. Jun, 2006 | Pubmed ID: 16406159 From Stationary to Instationary Metabolic Flux Analysis Advances in Biochemical Engineering/biotechnology. 2005 | Pubmed ID: 15791936 Investigating the Dynamic Behavior of Biochemical Networks Using Model Families Bioinformatics (Oxford, England). Apr, 2005 | Pubmed ID: 15604106 Serial 13C-based Flux Analysis of an L-phenylalanine-producing E. Coli Strain Using the Sensor Reactor Biotechnology Progress. May-Jun, 2004 | Pubmed ID: 15176872 New Tools for Mass Isotopomer Data Evaluation in (13)C Flux Analysis: Mass Isotope Correction, Data Consistency Checking, and Precursor Relationships Biotechnology and Bioengineering. Feb, 2004 | Pubmed ID: 14748080 Metabolic Isotopomer Labeling Systems. Part II: Structural Flux Identifiability Analysis Mathematical Biosciences. Jun, 2003 | Pubmed ID: 12711410 MMT--a Pathway Modeling Tool for Data from Rapid Sampling Experiments In Silico Biology. 2002 | Pubmed ID: 12611627 An Introduction to 13C Metabolic Flux Analysis Genetic Engineering. 2002 | Pubmed ID: 12416307 Modeling and Simulation: Tools for Metabolic Engineering Journal of Biotechnology. Mar, 2002 | Pubmed ID: 11792451 Общий протокол для оптимизации производства гетерологичных белка с использованием автоматизированной технологии Microbioreactor Johannes Hemmerich*1,2, Lars Freier*1,2, Wolfgang Wiechert1,2,3, Eric von Lieres1,2, Marco Oldiges1,2,4 1IBG-1: Biotechnology, Forschungszentrum Jülich, 2Research Center Jülich, Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 4Institute for Biotechnology, RWTH Aachen University JoVE 56234 Bio-ingénierie Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation JoVE 50560 Bio-ingénierie
Общий протокол для оптимизации производства гетерологичных белка с использованием автоматизированной технологии Microbioreactor Johannes Hemmerich*1,2, Lars Freier*1,2, Wolfgang Wiechert1,2,3, Eric von Lieres1,2, Marco Oldiges1,2,4 1IBG-1: Biotechnology, Forschungszentrum Jülich, 2Research Center Jülich, Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 4Institute for Biotechnology, RWTH Aachen University JoVE 56234 Bio-ingénierie
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation JoVE 50560 Bio-ingénierie