Laser Manipulation
Laser Manipulation - Related Videos
10:45
Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
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Cited by 2 •
2008
This video describes the manipulation of cultured neurons using laser tweezers in vitro.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
09:18
In vivo Laser Axotomy in C. elegans
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Cited by 46 •
2011
A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.
14:18
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
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Cited by 2 •
2016
A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.
05:54
Optogenetic Manipulation of Neural Circuits for the Sleep-to-Wakefulness Transition in Mice
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2025
Source: Kodani, S., et.al. Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. J. Vis. Exp. (2019)This video demonstrates the optogenetic manipulation of neural circuits involved in the sleep-to-wakefulness transition in mice. The procedure involves surgically implanting an optic fiber and electrodes into the brain and muscles to enable light stimulation and electrophysiological recording. By delivering light to activate light-sensitive cation...
FAQs
A tightly focused beam generates optical forces by transferring photon momentum to a microscopic object. For dielectric particles, this transfer can provide enough control to hold a target in place or move it through a small volume. The mechanism enables precise manipulation while preserving the important advantage of operating without direct physical contact.
Dielectric particles such as beads, cells, and organelles can respond to focused laser light through optical forces produced by photon-momentum transfer. Their suitability allows researchers to select biological objects for holding or movement in biochemistry experiments. This supports investigations of molecular forces, single-molecule behavior, and interactions involving cellular or subcellular structures.
Focusing the beam concentrates control at a small spatial scale, allowing researchers to position or move microscopic objects with high precision. This capability is especially useful when biochemical events occur in small volumes or involve individual molecules, cells, or organelles. The resulting physical control helps connect measured forces with molecular function.
Laser manipulation controls a target through optical forces rather than direct physical contact. That distinction allows beads, cells, or organelles to be held and moved while avoiding a mechanical probe touching the object. In biochemical research, noncontact control is useful for examining molecular forces and interactions where precise positioning is important.
A typical workflow uses a tightly focused laser beam to establish optical control over a suitable microscopic object, such as a bead, cell, or organelle. The object can then be positioned, held, or moved within a small volume. Researchers use that controlled state for microscale analysis, molecular-force measurements, or studies of biochemical interactions.
Researchers may choose this approach when an experiment requires precise control of microscopic objects without direct contact. Supported uses include single-molecule studies, measurement of molecular forces, cell and organelle handling, and microscale analysis of biochemical interactions. Its value is greatest when positioning or movement must be controlled within small volumes.
By controlling microscopic objects and measuring molecular forces, these experiments can connect physical behavior with molecular function. Single-molecule studies may use the controlled positioning of objects to examine biochemical interactions at very small scales. The resulting measurements provide a way to analyze how molecular activity relates to force and microscale behavior.
In biochemistry, precise optical control links physical measurements with molecular function and supports the handling of cells and organelles. These capabilities are relevant to diagnostics, drug development, and biotechnology, where microscale analysis and controlled investigation of biochemical interactions can provide useful research information. The technique therefore connects optical control with applied biological research.