Bacterial Artificial Chromosome
Bacterial Artificial Chromosome - Related Videos
03:06
Rescue of Infectious Virions from Cells Transfected with Bacterial Artificial Chromosome-Derived Synthetic RNA
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2026
Source: Yun, S. et al. Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses. J. Vis. Exp. (2015)This video demonstrates the recovery of infectious Japanese encephalitis virus from synthetic RNA transcribed from a bacterial artificial chromosome clone. It shows how cells are electroporated with the synthetic RNA, allowing the viral genome to direct the production of new virions. The resulting plaques formed in infected cell monolayers confirm...
12:20
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
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Cited by 5 •
2015
Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.
08:10
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
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Cited by 21 •
2019
The recent epidemic of Zika virus highlights the importance of establishing reverse genetic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious recombinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
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2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
08:21
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Cited by 11 •
2012
A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase.
FAQs
Its low-copy F-plasmid origin allows a very large DNA insert to replicate and remain stably inherited in Escherichia coli without relying on a high-copy plasmid arrangement. This feature is important when researchers need to maintain extensive pathogen or viral sequences as intact constructs for subsequent genetic analysis.
Unlike a system that breaks a locus into several smaller cloned pieces, a BAC can retain complete pathogen genes together with their regulatory regions. Keeping these neighboring sequences together helps investigators examine gene function in a more intact genetic context, which is relevant to virulence, host-pathogen interactions, and immune-evasion studies.
Selectable markers provide a practical way to identify Escherichia coli cells carrying the intended construct. In a BAC workflow, this selection distinguishes bacteria that have acquired the vector from those that have not, supporting recovery and maintenance of clones containing large pathogen-derived, regulatory, or viral DNA inserts.
Bacterial Artificial Chromosomes are particularly useful when the target is too large or context-dependent for fragmented cloning approaches. Supported examples include complete pathogen genes, their regulatory regions, and large viral genomes. Maintaining these sequences in one construct can preserve relationships that are difficult to study when genetic material is separated into smaller pieces.
In immunology and infection research, BACs provide constructs for analyzing how pathogen or viral genetic content contributes to disease-related traits and interactions with host defenses. Researchers can use them to investigate virulence, host-pathogen interactions, and immune evasion. The resulting genetic context also informs efforts to identify vaccine or therapeutic targets.
By preserving large genomic segments, BAC-based studies can connect sequence content with regulatory organization rather than examining an isolated gene alone. This is valuable when interpreting pathogen biology or viral genome function because the construct retains broader genetic context for analysis. Such information can guide mechanistic studies and development of vaccine or therapeutic targets.