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Journal / Immunology and Infection / Bacterial Artificial Chromosomes: A Functional…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Bacterial Artificial Chromosomes
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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Article DOI: 10.3791/53164 • 12:21 min
December 29th, 2015 •
1, 1, 1, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah Science Technology and Research, College of Agriculture and Applied Sciences, Utah State University

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December 29th, 2015

Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.

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