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JoVE Journal
Immunology and Infection

This content is Open Access.

Bacterial Artificial Chromosomes
 

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Article DOI: 10.3791/53164
December 29th, 2015

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Summary December 29th, 2015

Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.

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