Murine Surgery
Murine Surgery - Related Videos
04:36
Murine Superficial Lymph Node Surgery
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Cited by 10 •
2012
To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.
07:32
Murine Flexor Tendon Injury and Repair Surgery
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Cited by 45 •
2016
Flexor tendons in the hand are commonly injured, leading to impaired hand function. However, the scar-tissue healing response is not well characterized. A murine model of flexor tendon healing is demonstrated here. This model can enhance overall understanding of the healing process and assess therapeutic approaches to improve healing.
07:34
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
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2021
Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.
12:13
Considerations for Rodent Surgery
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including patient preparation, surgeon preparation, sterilization of instruments and other supplies, and the use of a clean and controlled environment. Presurgical planning,...
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
FAQs
Anesthesia permits the planned operation, while analgesia supports recovery and helps limit procedure-related inflammation. This distinction matters in immunology experiments because inflammation caused by the operation itself can affect measurements of host responses. Consistent use of these measures therefore supports animal welfare while improving the reproducibility of comparisons among experimental groups.
Aseptic technique and deliberate closure help keep the operative procedure controlled from incision through recovery. Together with postoperative monitoring, they support consistent healing and reduce procedure-related influences that could complicate interpretation. Maintaining these conditions is especially important when researchers are measuring immune responses, pathogen effects, or changes in tissue and organ function.
Each operative action creates a defined relationship between the tissue being studied and the immune response being measured. Manipulation or removal can model tissue injury or organ dysfunction, whereas sampling provides material for assessing local or systemic effects. Researchers must therefore interpret findings in relation to both the target tissue and the surgical intervention.
A controlled workflow begins with anesthesia and aseptic preparation, followed by a planned incision and the specified manipulation, removal, or tissue sampling. The operative site is then closed, analgesia is provided, and the animal is monitored during recovery. Keeping these stages consistent helps preserve welfare and experimental reproducibility across procedures.
Researchers use these procedures when a study requires controlled access to tissues or an experimental model of tissue injury, organ dysfunction, infection, or immune challenge. The approach can support measurement of host responses, pathogen effects, and therapeutic outcomes. It also enables examination of how local tissue events relate to systemic immunity.
These models can generate information about responses at the site of tissue intervention as well as effects throughout the organism. Depending on the procedure, investigators can examine tissue injury, organ dysfunction, pathogen effects, immune responses, or therapeutic outcomes. Standardized operations make it easier to compare these outcomes across experimental conditions while limiting procedure-related variation.