Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Engineering / Surgery and Sample Processing for Correlative…
JoVE Journal
Engineering

A subscription to JoVE is required to view this content.

JoVE Journal Engineering
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
Journal (Engineering)
Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy
Journal (Engineering)
Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy
 

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Article DOI: 10.3791/62581-v • 07:34 min • August 5th, 2021
August 5th, 2021 •
1, 2, 2, 3, 3, 3, 2, 1
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, 2Center for Regenerative Medicine, Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Chapters

Summary
Automatic Translation

Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

Tags

Surgery Sample Processing Correlative Imaging Murine Pulmonary Valve Heterogeneity Controlled Fixation High-resolution Images Anatomical Location Procedure Dr. Tai Yi Microsurgery Regenerative Medicine Autoclaving Tools Mouse Dissection Fine Scissors Microforceps Microvascular Clamps Clamp Applying Forceps Microneedle Holders Spring Scissors Retractors Euthanizing Mouse Dorsal Recumbent Position Thoracotomy Heart Exposure Adipose Tissue Removal Fascia Removal Saline Solution Perfusion Heart Removal Ventriculoatrial Junction Severing
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter