Tumor Disaggregation
Tumor Disaggregation - Related Videos
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
0 Views •
Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
13:25
Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
0 Views •
Cited by 2 •
2022
This paper details methods that demonstrate three common biofilm harvesting and disaggregation techniques on two surface types, ruggedness testing of a harvesting method and minimum information to consider when choosing and optimizing harvesting and disaggregation techniques to increase reproducibility.
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
0 Views •
Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
07:55
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
0 Views •
Cited by 8 •
2011
A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.
11:09
Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
0 Views •
Cited by 104 •
2010
Luciferase-modified human brain tumor xenografts can be established intracranially in athymic mice, with subsequent monitoring of tumor growth and response to therapy using bioluminescence imaging. In combination with survival analysis, bioluminescence monitoring is an essential research tool for pre-clinical testing of therapies being considered for treating brain tumors.
FAQs
Mechanical disruption and enzymatic digestion contribute different but complementary actions. Mechanical force breaks tissue apart, while enzymes loosen the extracellular matrix and cell-cell attachments. Using both processes helps release cells or small clusters from the solid structure. This combined approach creates material that can be directed toward cellular analysis while retaining attention to viability.
Temperature, exposure time, and physical handling influence whether released cells remain viable. Conditions that are not adequately controlled can reduce the quality of the resulting cellular material, limiting its usefulness for culture, profiling, or testing. Careful control is therefore essential when preparing a suspension intended for downstream medical research or patient-derived models.
The desired suspension format depends on the downstream question. Individual cells make cellular populations more accessible for measurements such as flow cytometry, whereas small clusters provide partially grouped material when complete separation is unnecessary. This choice matters because tumor tissue is heterogeneous, so disaggregation should produce material suited to the intended analysis without treating every sample as biologically uniform.
A practical workflow brings tumor tissue through mechanical disruption and enzymatic digestion under controlled conditions, then yields a suspension of individual cells or small clusters. Temperature, enzyme exposure time, and physical handling require attention throughout because they affect cell viability. The resulting preparation can then be directed toward an analytical, culture, organoid, or drug-response application.
Once generated, the suspension provides access to cells for several readouts. Flow cytometry can support characterization of cellular populations, while genomic and molecular profiling examines tumor-related features in the prepared material. Drug-response testing uses the accessible cells or clusters to evaluate treatment effects. Together, these outputs connect sample composition with experimental and translational research questions.
Patient-derived cultures and organoids are important uses when researchers need models established from a tumor sample. Disaggregation supplies the cellular starting material for these systems, allowing investigators to study cancer populations in a model derived from patient material. These models can support drug-response testing and contribute to more representative research platforms for precision medicine.
By making heterogeneous tumor material accessible, Tumor Disaggregation supports investigation of distinct cancer populations and treatment resistance. It also contributes to precision medicine by providing material for molecular characterization, drug-response testing, and patient-derived models. In medicine-focused research, its value lies in linking the cellular diversity of a tumor with questions about how disease responds to therapy.