Primary Mouse Neurons
Primary Mouse Neurons - Related Videos
11:58
Primary Culture of Mouse Dopaminergic Neurons
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Cited by 66 •
2014
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.
01:59
Modeling Neuronal Injury in Mouse Primary Cerebellar Granule Neurons
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2025
This video demonstrates the generation of a neuronal injury model in mouse primary cerebellar granule neurons. It outlines the steps involved in inducing neuronal injury using NMDA, an excitatory neurotransmitter receptor agonist, and glycine, a co-agonist. NMDA and glycine bind to specific neuronal receptors, causing their overstimulation and resulting in a massive intracellular calcium influx, which leads to neuronal cell damage and death.
10:36
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
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Cited by 2 •
2017
This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.
04:04
Isolation and Culture of Primary Embryonic Mouse Midbrain Dopamine Neurons
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2025
The video demonstrates the process of isolating and culturing dopamine neurons from the midbrain of mouse embryos. The isolated ventral midbrain floors are dissociated using enzymes. The resulting dopamine neurons are harvested and transferred into micro-islands inside multi-well plates. The cells are incubated with a dopamine neuron medium to maintain the cells at a high density inside the micro-islands.
08:43
Primary Neuronal Cultures
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2023
The complexity of the brain often requires neuroscientists to use a simpler system for experimental manipulations and observations. One powerful approach is to generate a primary culture by dissecting nervous system tissue, dissociating it into single cells, and growing those cells in vitro. Primary cultures make neurons and glia easily accessible to the experimental tools required for techniques like genetic manipulation and time-lapse imaging. Furthermore, these cultures represent a highly...
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After tissue dissociation, the cells must attach to a culture substrate and remain under defined conditions that support neurite extension. As neurites grow, they establish interconnected networks capable of electrical signaling and synaptic communication. These features make the culture useful for examining how environmental conditions affect neuronal structure and the development of network-level function.
Primary mouse neurons preserve many features of differentiated neurons, allowing experiments to connect cellular changes with nervous-system function. Their neuronal structure supports analysis of axon and dendrite growth, while their communication properties permit investigation of electrical signaling and synaptic interactions. This biological context helps researchers interpret cellular responses beyond what engineered cell systems may show.
These cultures provide an experimentally accessible setting between intact animal studies and engineered cell systems. Researchers can examine neuronal structure, gene expression, signaling, and responses to treatment in isolated cells while retaining characteristics of differentiated neurons. The resulting observations can connect cellular mechanisms with broader nervous-system function and complement, rather than replace, studies in animals or engineered models.
The preparation begins with brain or spinal tissue, which is dissociated to separate cells for culture. The resulting cells are placed on a culture substrate, where they attach and extend neurites under defined conditions. With continued growth, the neurites form networks that support studies of neuronal development, cellular communication, and changes caused by experimental treatments or injury.
Primary mouse neurons support investigations of neuronal development, axon and dendrite growth, gene expression, and responses to drugs or injury. Because several of these measurements can be examined in the same general culture model, researchers can relate structural changes to molecular or functional outcomes. This makes the system relevant to diverse questions in cellular and systems neuroscience.
Researchers can evaluate how neurons develop their processes, how gene expression changes, and how cells respond to drugs or injury. The cultures also support examination of electrical signaling and synaptic communication after networks form. Together, these outcomes provide cellular-level evidence about neuronal structure and function and help relate experimental perturbations to nervous-system mechanisms.