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Detektion og Genogrouping af norovira fra børne Taburetter Ved Taqman Et-trins RT-PCR

Article DOI: 10.3791/3232-v • 15:17 min • July 22nd, 2012
July 22nd, 2012 •
1, 1, 1,2, 3, 3, 1, 1, 2, 1, 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

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En One-Step RT-PCR assay til detektion og genogruppe identifikation af norovirus isolater fra børns afføring, som udnytter primere og TaqMan prober specifikke for den åbne læseramme 1 (ORF1)-ORF2 forbindelsesregionen, mest konserverede region i norovirus genomet er beskrevet. En ikke-kommercielle, omkostningseffektive RNA ekstraktion metode er nærmere beskrevet.

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Virology Medicine Genetics norovirus gastroenteritis RNA-ekstraktion diarré afføringsprøver PCR RT-PCR TaqMan silica
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