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Journal / Immunology and Infection / 肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集…
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Immunology and Infection

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JoVE Journal Immunology and Infection
肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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Journal (Immunology and Infection)
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肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集

Article DOI: 10.3791/4420-v • 13:58 min • October 22nd, 2012
October 22nd, 2012 •
1, 1
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

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此协议描述了使用的肽：MHC四聚体和磁性微珠分离低频表位特异性的T细胞群体，并通过流式细胞术分析它们。这种方法能够直接研究内源性T细胞群的兴趣

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免疫学，分子生物学，细胞生物学，第68，T细胞，T细胞受体，四，流式细胞仪检测，抗原特异性，免疫学，免疫反应，磁性，丰富，
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