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Single-molekyle Imaging af genregulering

Article DOI: 10.3791/50042-v • 11:31 min • March 15th, 2013
March 15th, 2013 •
1, 1,2,3, 1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University

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Vi beskriver en fluorescensmikroskopi fremgangsmåde, cotranslationel aktivering ved spaltning (CoTrAC), til billede produktionen af ​​proteinmolekyler i levende celler med enkelt-molekyle præcision uden at forskubbe proteinets funktionalitet. Denne fremgangsmåde er blevet anvendt til at følge de stokastiske udtrykket dynamik en transkriptionsfaktor, at λ repressor CI

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Biofysik Biokemi Genetik kemi molekylærbiologi cellebiologi mikrobiologi Proteins enkelt molekyle fluorescens protein protein ekspression cotranslational aktivering CoTrAC cellekultur fluorescerende mikroskopi billedbehandling translational aktivering systembiologi
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