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立体定向注射的microRNA表达慢病毒的小鼠海马CA1区和行为结果的评估

Article DOI: 10.3791/50170-v • 09:06 min • June 10th, 2013
June 10th, 2013 •
1, 1, 1
1Department of Biological Chemistry and The Edmond & Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem

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小分子RNA在大脑的结构和功能有显着的作用。在这里，我们描述了一种方法来执行海马miRNA的过表达一个精心设计的miRNA表达慢病毒采用立体定向注射。这种方法可以作为一个相对快速的方式评估

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行为期76，神经学，神经生物学，解剖学，生理学，生物医学工程，生物工程，分子生物学，外科，神经系统疾病，核酸，核苷酸，核苷，行为测试，海马，学习和记忆，小分子RNA，立体注射，莫里斯水迷宫，鼠标，动物模型;
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