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Naive Adult Stem Cells Isolasjon fra primære humane fibroblastkulturer

Article DOI: 10.3791/50185-v • 07:39 min • May 3rd, 2013
May 3rd, 2013 •
1, 1, 2, 1
1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München

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Vi rapporterer en metode for å isolere naive multipotent hud-derived forløper (SKP) celler fra primære humane fibroblastkulturer. Vi viser at disse sKPs avledet fra fibroblastkulturer dele lignende stamcelleegenskaper til de som stammer direkte fra menneskelig hud biopsier. Disse cellene uttrykke neural crest markør, nestin, i tillegg til de multipotent markører som OCT4 og Nanog.

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Stem Cell Biology cellebiologi molekylær biologi anatomi fysiologi Biomedical Engineering medisin dermatologi Cells kulturperler stamceller biologi (generelt) Hud og bindevevssykdommer Biologiske fenomener voksne stamceller hud avledet forløper celler fibroblaster sfære kultur hud-deriverte forløpere SKP PCR qPCR immunocytochemistry isolasjon cellekultur
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