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Immunology and Infection

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JoVE Journal Immunology and Infection
Vurdere utvikling av Murint Plasmacytoid dendrittiske celler i Peyer sin Patches Bruke Adoptive Overføring av Hematopoietic stamfedre
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Vurdere utvikling av Murint Plasmacytoid dendrittiske celler i Peyer sin Patches Bruke Adoptive Overføring av Hematopoietic stamfedre

Article DOI: 10.3791/51189-v • 13:34 min • March 17th, 2014
March 17th, 2014 •
1, 1,2
1Department of Immunology, The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

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Denne protokollen beskriver eksperimentelle prosedyrer for å vurdere differensiering av plasmacytoid dendrittiske celler i Peyer patch fra vanlige dendrittiske cellen stamfedre, ved hjelp av teknikker som involverer FACS-mediert celle isolasjon, hydrodynamisk genoverføring, og strømningsanalyse av immun undergrupper i Peyer patch.

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Immunologi hematopoiesen dendrittiske celler Peyer patch cytokiner adoptiv overføring
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