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Tilpasning af halvautomatiseret Cirkulerende Tumor Cell (CTC) analyser til kliniske og prækliniske forskning applikationer

Article DOI: 10.3791/51248-v • 14:14 min • February 28th, 2014
February 28th, 2014 •
1,2, 3, 3,4, 1,2,4,5
1London Regional Cancer Program, London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry, Western University, 3Special Hematology/Flow Cytometry, London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology, Western University

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Cirkulerende tumorceller (CTCs) er prognostisk i flere metastatiske cancere. Dette håndskrift beskriver guldstandarden CellSearch systemet (CSS) CTC tælling platform og fremhæver fælles misklassifikation fejl. Desuden er to tilpassede protokoller beskrevet for brugerdefineret markør karakterisering af CTCs og CTC optælling i prækliniske musemodeller af metastaser ved hjælp af denne teknologi.

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Medicine metastase cirkulerende tumorceller (CTCs) CellSearch systemet brugerdefineret markør karakterisering, Præklinisk musemodel klinisk forskning
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