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JoVE Journal Chemistry
Kvantitative Proteomikk Bruke Reduktiv Dimethylation for stabile isotoper Merking
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Kvantitative Proteomikk Bruke Reduktiv Dimethylation for stabile isotoper Merking

Article DOI: 10.3791/51416-v • 11:53 min • July 1st, 2014
July 1st, 2014 •
1,2,3, 4
1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center

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Stabile isotoper merking av peptider ved reduktiv dimethylation (Redi merking) er en rask, billig strategi for nøyaktige massespektrometri-basert kvantitative proteomikk. Her vi viser en robust fremgangsmåte for fremstilling og analyse av proteinblandinger ved hjelp av REDI tilnærming som kan brukes på nesten hvilken som helst prøvetype.

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Kjemi kvantitative proteomikk massespektrometri stabile isotoper reduktiv dimethylation peptid merking LC-MS/MS
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