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Journal / Biology / siRNA的筛选识别泛素和泛素样系统压力表生物学途径在培养的哺乳动物细胞…
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siRNA的筛选识别泛素和泛素样系统压力表生物学途径在培养的哺乳动物细胞
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siRNA的筛选识别泛素和泛素样系统压力表生物学途径在培养的哺乳动物细胞

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

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在这里，我们描述了一种方法来执行对象的siRNA“ubiquitome”屏幕来识别对缺氧的HIF1A介导的细胞应答的新型泛素和泛素样调节剂。这可以适用于任何生物学途径，其中一个强大的读出报告基因活性的是可用的。

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生物化学，第87，siRNA的筛选，泛素，UBL，ubiquitome，缺氧，HIF1A，高通量，哺乳动物细胞，荧光素酶报道
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