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Ikke-enzymatisk, Serum-free Tissue Culture of Pre-invasive brystlesjonene for Spontan generasjon Mammospheres

Article DOI: 10.3791/51926-v • 09:49 min • November 8th, 2014
November 8th, 2014 •
1, 2, 1
1Center for Applied Proteomics and Molecular Medicine, George Mason University, 2Virginia Surgery Associates

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Primære cellekultur ved hjelp av intakte vev organoids gir et modellsystem som etterligner multi-mobilnettet in vivo mikromiljøet. Vi utviklet et serum-free primære brystepitel vevskultur modell som foreviger blandede cellekultur linjene og utstillinger differensierte morfologi, uten enzymatisk vev avbrudd. Bryst organoids være levedyktig i> 6 måneder.

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Cancer Biology bryst duktalt carcinoma in situ epidermal vekstfaktor mammosphere organoid pre-invasive primærcellekultur serum-fri spheroid
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