Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.