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Immunology and Infection

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调查肥大细胞分泌颗粒;从合成到胞吐作用
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调查肥大细胞分泌颗粒;从合成到胞吐作用

Article DOI: 10.3791/52505-v • 16:01 min • January 26th, 2015
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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本议定书的目标是开发一种方法，使肥大细胞分泌功能基因组分析。所述协议是基于荧光报道基因cotrasfected与感兴趣和实时的基因的释放定量评估分析分泌颗粒的形态。

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免疫学，第95，肥大细胞，胞外分泌，分泌颗粒，活细胞成像，Rab5的，报告基因，共焦成像，转
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