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Plasmide dérivé d'ADN Strand Displacement Gates Réseaux de réaction d'exécution chimiques
Journal JoVE
Biologie
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Journal JoVE Biologie
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmide dérivé d'ADN Strand Displacement Gates Réseaux de réaction d'exécution chimiques

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07:50 min

November 25, 2015

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07:50 min
November 25, 2015

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Summary

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This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

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