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Developmental Biology

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JoVE Journal Developmental Biology
Isolierung von Perivaskuläre multipotenten Vorläuferzellpopulationen aus menschlichem Herzgewebe
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Isolierung von Perivaskuläre multipotenten Vorläuferzellpopulationen aus menschlichem Herzgewebe

Article DOI: 10.3791/54252-v • 08:15 min • October 8th, 2016
October 8th, 2016 •
1, 2,3, 4, 4, 4, 5,6, 4,7
1Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine, University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 6Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

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Menschliche Herzgewebe birgt multi perivaskuläre Vorläuferzellpopulationen, die für myokardiale Regeneration geeignet sein können. Die hier beschriebene Technik ermöglicht die gleichzeitige Isolierung und Reinigung von zwei multi stromalen Zellpopulationen , die mit nativem Blutgefäße, dh CD146 + CD34 - Perizyten und CD34 + CD146 - Adventitia - Zellen aus dem menschlichen Herzmuskel.

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Entwicklungsbiologie Heft 116 pericyte adventitiellen Zelle Blutgefäß Stammzellen Vorläuferzellen Herzvorläuferzelle Myokard Herzregeneration Durchflusszytometrie
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