Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Utnytte pHluorin-merket reseptorer å overvåke…
JoVE Journal
Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Biology
Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
Journal (Biology)
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
Journal (Biology)
Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
 
Click here for the English version

Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel

Article DOI: 10.3791/55466-v • 09:59 min • March 16th, 2017
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Merking av ekstracellulære domenet til en membran protein med en pH-sensitive fluorofor, superecliptic pHluorin (SEP), kan subcellulære lokalisering, uttrykk og menneskehandel skal fastsettes. Imaging september-merkede proteiner med total indre refleksjon fluorescens mikroskopi (TIRFM) muliggjør kvantifisering av proteinnivåer i den perifere ER og plasmamembran.

Tags

Biofysikk Superecliptic pHluorin reseptor membran trafficking subcellulære lokalisering plasma membran innsetting total intern refleksjon fluorescens mikroskopi membran protein oppregulering
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter